Data/hora: 19/05/2024 04:20:04

Provedor de dados: 	83	
País: 	Brazil	
Título: 	Microsatellite loci development for three catfish species from northwestern South America	
Autores: 	Restrepo-Escobar,Natalia; Márquez,Edna J.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Freshwater fish; Molecular markers; Next-generation sequencing; Siluriformes.	
Resumo: 	ABSTRACT The Neotropical catfish species Ageneiosus pardalis, Pimelodus grosskopfii, and Sorubim cuspicaudus are important fishery resources in Colombia that show historical declines in their capture. This study used next-generation sequencing with 454 FLX technology (Roche Applied Science) and bioinformatics analysis to develop between 18 and 24 microsatellite loci for these species. The novel microsatellite loci showed high values of polymorphic information content -PIC (A. pardalis: 0.601-0.903, P. grosskopfii: 0.748-0.946 and S. cuspicaudus: 0.383-0.876), and the average number of alleles/locus ranged from 7-15 for A. pardalis, 9-30 for P. grosskopfii and 5-14 for S. cuspicaudus. The average observed and expected heterozygosities were respectively, 0.757 ± 0.035 and 0.834 ± 0.015 for A. pardalis; 0.596 ± 0.040 and 0.881 ± 0.009 for P. grosskopfii; and 0.747 ± 0.031 and 0.757 ± 0.025 for S. cuspicaudus. For future studies, these loci can be useful to estimate the genetic diversity and population structure in these three Neotropical catfishes.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000100210	
Editor: 	Sociedade Brasileira de Ictiologia	
Relação: 	10.1590/1982-0224-2019-0079	
Formato: 	text/html	
Fonte: 	Neotropical Ichthyology  v.18 n.1 2020	
Fonte: 	info:eu-repo/semantics/openAccess